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Sydney NSW, Australia
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2017-11-15T04:00:00.0000000Z
Fig. 3 Disease incidence and gene expression analysis. a, b Levels of Foc TR4 infection in selected transgenic and WT banana plants throughout the 3-year field trial. a WT and RGA2 lines, and b WT and Ced9 lines. Wet seasons (November–April) are indicated. The number of biological replicates (n) of each independent line at the start of the trial is provided in Supplementary Table 1. Data points are percentage of biological replicates infected at time of assessment. *0.01 < p < 0.05, **0.001 < p < 0.01 at trial end (Tukey's HSD test). c, d Analysis of RGA2 mRNA expression levels in transgenic and WT banana plants. c Analysis of transgene (RGA2-Nos) expression levels using primers designed to amplify a 96 bp fragment spanning the RGA2transgene/Nos terminator junction. d Analysis of RGA2 transgene and endogenous mRNA expression levels using primers designed to amplify a 92 bp fragment of both the RGA2transgene and RGA2 endogenous sequences. All values are normalized expression levels expressed relative to line RGA2-3. WT GN; TR4-susceptible M. acuminata ssp. malaccensis(Mal-S) and TR4-resistant M. acuminata ssp. malaccensis (Mal-R). A single biological replicate was analyzed with three technical replicates (n = 3). Data are mean ± SEM Table 1. DPM25, Dwarf Parfitt Mutant; GCTCV, Giant Cavendish Tissue Culture Variants; GN, Grand Nain; HSD, honest significant difference; TR4, tropical race 4. Data are percentage of biological replicates infected at time of assessment. *0.01 < p < 0.05, **0.001 < p < 0.01 at trial end (Tukey's HSD test). Fig. 1 Transgene expression cassettes and Southern analysis of selected transgenic lines. a RGA2 and b Ced9 expression cassettes. LB, left border; RB, right border. Determination of transgene copy number in c RGA2 and d Ced9 transgenic banana lines by Southern blot analysis. Genomic DNA from WT, RGA2 and Ced9 lines was digested with HindIII and XmaI, respectively. DNA molecular weight marker II (Roche) reference is indicated on the right hand side Protein sequence alignment of the RGA2 transgene sequence from TR4-resistant M. acuminata ssp. malaccensis (Mal-R) and the three consensus RGA2 homologous sequences from WT GN. Amino acid differences are highlighted Fig. 2 Characteristic symptoms of Foc TR4 in susceptible and resistant banana. External symptoms and reddish-brown internal vascular discoloration of Foc TR4 in infected WT Cavendish a and b compared with resistant transgenic lines RGA2-3 c and d, and Ced9-21 eand f

Following the note sent out yesterday on the work at QUT (Queensland University of Technology) Amit Sukal has kindly sent me a link to a paper in Nature Communciation https://www.nature.com/ articles/s41467-017-01670-6

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  • James Dale,  …
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    banana
    Fusarium_TR4
    Panama
    University_of_Queensland
    Cavendish
    Transgenic
    Nematode_Caenorhabditis_elegans
    Fusarium_oxysporum_f_sp_cubense
    Sydney NSW, Australia
    For your information
    2017-11-15T04:00:00.0000000Z
    CRISPR/Cas9-mediated disruption of genes associated with cuticle pigment caused mosquitoes to turn from black to yellow, and disruption of genes associated with eye pigment caused eye color to change from black to white

    EurekAlert https://eurekalert.org/pub_releases/2017-11/uoc--mms111417.php

    UCR researchers are generating genetically engineered insects to help prevent the spread of infectious diseases

    University of California, Riverside As proof of concept, the …

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    Gene_drive
    Mosquitoes
    Cas9
    Aedes_aegypti
    Sydney NSW, Australia
    For your information
    2017-11-15T04:00:00.0000000Z

    I have been sent a poster by Frank Visser produced by Catherine Hardiman entitled: Ant toxin: A strategy for the indirect control of mealybug, vector of Grapevine leafroll-associated virus type 3 (GLRaV-3).  This is attached There is a …

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    Mealybug
    Grapes
    Ant_toxin
    Bifenthrin
    Sydney NSW, Australia
    For your information
    2017-11-14T19:00:00.0000000Z
    The tortoise beetle, which eats thistle leaves, has evolved a symbiotic relationship with bacteria that allows it to have such a specialized diet. Credit: Photo by Hassan Salem

    The specialized diet of a beetle is largely due to bacteria that live inside the insect

    Summary:
    A leaf-eating beetle has evolved a symbiotic relationship that allows the …
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    Symbiosis
    Beetles
    Bacteria
    Cassida_rubiginosa
    Tortoise_beetle
    Degrade_pectin
    Sydney NSW, Australia
    For your information
    2017-11-14T18:00:00.0000000Z
    For all the damage that aphids can cause (such as these tulip bulb aphids, Dysaphis tulipae), they need a partner to help them extract nutrients from the plant sap they feed on. That partner is a bacterial symbiont called Buchnera that lives in the aphid’s digestive system. (Photo credit: Whitney Cranshaw, Colorado State University, Bugwood.org)

    Entomology Today https://entomologytoday.org/2017/11/15/nutritional-symbionts-why-some-insects-dont-have-to-eat-their-vegetables/

    By Lora Kraft Broccoli was the main vegetable at my family’s house growing up. Despite my sisters’ and my distaste for …

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    Symbiosis
    Termites
    Cellulose
    Wolbachia
    B_vitamins
    Buchnera
    Sydney NSW, Australia
    For your information
    2017-11-14T18:00:00.0000000Z

    ResearchInGermany http://www.research-in-germany.org/en/research-landscape/news/2017/11/2017-11-14-newly-discovered-fungus-feeds-phosphorus-to-plants.html University of Cologne

    Researchers from the University of Cologne discovered a fungus that …
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    Mycorrhizal_symbiosis
    University_of_Cologne
    Fungal_microbiome
    Phosphorus
    Sydney NSW, Australia
    For your information
    2017-11-14T04:00:00.0000000Z

    Crop Biotech Update, sponsored by commercial companies, has several articles on GM crops that may be of interest to members

    A large, prospective cohort study conducted among agricultural …
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    Photosynthesis
    Golden_potatoes
    Glyphosate_cancer
    Sydney NSW, Australia
    For your information
    2017-11-14T04:00:00.0000000Z
    FreshFruitPortal https://www.freshfruitportal.com/news/2017/11/15/australian-university-develops-bananas-tr4-resistance/ The same Australian university that is trialing Vitamin A-enriched bananas in Uganda has successfully developed …
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    banana
    Panama_disease
    Vitamin_A
    Fusarium_TR4_resistance
    Sydney NSW, Australia
    For your information
    2017-11-14T04:00:00.0000000Z

    The Guardian https://www.theguardian.com/commentisfree/2016/oct/25/banana-farming-danger-cavendish-crop-genetics

    By Ioannis Stergiopoulos, André Drenth and Gert Kema

    The banana is the world’s most popular fruit crop, with over 100m …

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    banana
    Fusarium_TR_
    Cavendish
    Gros_Michel
    Black_Sigatoka
    Pseudocercospora_fijiensis
    Sydney NSW, Australia
    For your information
    2017-11-14T04:00:00.0000000Z

    The Guardian https://www.theguardian.com/environment/2016/apr/21/banana-fungal-disease-threat-latin-americaBy Oliver Milman Banana experts from around the world have gathered in Florida to find a way to halt a disease that is wiping out the …

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    banana
    Fusarium_wilt
    FAO
    Fusarium_TR4
    Panama_disease
    Cavendish